Bioinformatics tools for phase seperation

Last updated on Nov 5, 2025
By Chao Hou

I have developed several bioinformatics tools for studying phase separation: PhaSepDB: A curated database that provides a collection of liquid-liquid phase separation (LLPS) proteins and proteins located in membraneless organelles (MLOs).
MloDisDB: A curated database that provides the relationships between membraneless organelles and diseases.
PhaSePred: A meta-predictor for LLPS proteins, distinguishing LLPS proteins as self-assembling or partner-dependent. It integrates results from tools like catGRANULE, PScore, PLAAC, ESpritz, SEG, CIDER, DeepCoil, and DeepPhase.
Additionally, I designed experiments to investigate the phase separation properties of chromatin-associated proteins using gradient elution with 1,6-hexanediol.

Phase Seperation
Chao Hou
Chao Hou
PhD of Bioinformatics

My research interest is AI4biology.

Publications

Investigating phase separation properties of chromatin-associated proteins using gradient elution of 1,6-hexanediol
Chromatin-associated phase separation proteins establish various biomolecular condensates via liquid-liquid phase separation (LLPS), …
Peiyu Zhu, Chao Hou, Manlin Liu, Taoyu Chen, Tingting Li, Likun Wang
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Investigating phase separation properties of chromatin-associated proteins using gradient elution of 1,6-hexanediol
PhaSepDB in 2022: annotating phase separation-related proteins with droplet states, co-phase separation partners and other experimental information
Phase separation (PS) proteins form droplets to regulate myriad membraneless organelles (MLOs) and cellular pathways such as …
Chao Hou, Xinxin Wang, Haotai Xie, Taoyu Chen, Peiyu Zhu, Xiaofeng Xu, Kaiqiang You, Tingting Li
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PhaSepDB in 2022: annotating phase separation-related proteins with droplet states, co-phase separation partners and other experimental information
Screening membraneless organelle participants with machine-learning models that integrate multimodal features
Protein self-assembly is one of the formation mechanisms of biomolecular condensates. However, most phase-separating systems (PS) …
Zhaoming Chen, Chao Hou, Liang Wang, Chunyu Yu, Taoyu Chen, Boyan Shen, Yaoyao Hou, Pilong Li, Tingting Li
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Screening membraneless organelle participants with machine-learning models that integrate multimodal features
MloDisDB: a manually curated database of the relations between membraneless organelles and diseases
Cells are compartmentalized by numerous membrane-bounded organelles and membraneless organelles (MLOs) to ensure temporal and spatial …
Chao Hou, Haotai Xie, Yang Fu, Yao Ma, Tingting Li
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MloDisDB: a manually curated database of the relations between membraneless organelles and diseases