Bioinformatics tools for phase seperation

Last updated on Sep 17, 2023
By Chao Hou

I’ve developped three bioinformatics tools for studying phase separation: PhaSepDB is a novel database that provides a collection of manually curated liquid-liquid phase separation (LLPS) proteins and Membraneless organelles (MLOs) located proteins. MloDisDB is a manually curated dataBase of the relations between membraneLess organelles and diseases PhaSePred is A Meta-predictor For Phase-Separating Proteins. We define and predict the LLPS proteins as self-assemble and partner-dependent, the results from catGRANULE, PScore, PLAAC, ESpritz, SEG, CIDER, DeepCoil, DeepPhase were integrated as well.

I also designed experiments to investigate phase separation properties of chromatin-associated proteins using gradient elution of 1,6-hexanediol.

Phase Seperation
Chao Hou 侯超
Chao Hou 侯超
PhD of Bioinformatics

My research interests include computational biology and genetics.

Publications

Investigating phase separation properties of chromatin-associated proteins using gradient elution of 1,6-hexanediol
Chromatin-associated phase separation proteins establish various biomolecular condensates via liquid-liquid phase separation (LLPS), …
Peiyu Zhu, Chao Hou 侯超, Manlin Liu, Taoyu Chen, Tingting Li, Likun Wang
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Investigating phase separation properties of chromatin-associated proteins using gradient elution of 1,6-hexanediol
PhaSepDB in 2022: annotating phase separation-related proteins with droplet states, co-phase separation partners and other experimental information
Phase separation (PS) proteins form droplets to regulate myriad membraneless organelles (MLOs) and cellular pathways such as …
Chao Hou 侯超, Xinxin Wang, Haotai Xie, Taoyu Chen, Peiyu Zhu, Xiaofeng Xu, Kaiqiang You, Tingting Li
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PhaSepDB in 2022: annotating phase separation-related proteins with droplet states, co-phase separation partners and other experimental information
Screening membraneless organelle participants with machine-learning models that integrate multimodal features
Protein self-assembly is one of the formation mechanisms of biomolecular condensates. However, most phase-separating systems (PS) …
Zhaoming Chen, Chao Hou 侯超, Liang Wang, Chunyu Yu, Taoyu Chen, Boyan Shen, Yaoyao Hou, Pilong Li, Tingting Li
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Screening membraneless organelle participants with machine-learning models that integrate multimodal features
MloDisDB: a manually curated database of the relations between membraneless organelles and diseases
Cells are compartmentalized by numerous membrane-bounded organelles and membraneless organelles (MLOs) to ensure temporal and spatial …
Chao Hou 侯超, Haotai Xie, Yang Fu, Yao Ma, Tingting Li
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MloDisDB: a manually curated database of the relations between membraneless organelles and diseases